Textile tissue engineering: a path towards organ weaving

Abstract

Event Abstract Back to Event Textile tissue engineering: a path towards organ weaving Ali Tamayol1, Iman K. Yazdi1, Afsoon Fallahi1, Mahboubeh Nabavinia1, Huseyin Avci1, Raquel Costa-Almeida1, Grissel T. Santiago1, Mario M. Alvarez1, Mohsen Akbari1, Nasim Annabi1 and Ali Khademhosseini1 1 Brigham and Women's Hospital, Medicine, United States Introduction: Textile technologies have opened a new area in tissue engineering. Precise control over the distribution of different cell types and microstructure of fabricated constructs are considered as key advantages of textile techniques. Insufficient mechanical properties of cell-carrying fibers made of hydrogels have limited their usage in textile processes. Thus, the concept of composite living fibers (CLFs) that can withstand textile processing has been recently introduced to address this challenge. However, these CLFs could not support long term cellular viability and functionality. Here we introduced the hybrid bioactive hydrogels for fabrication of the new generation of CLF that not only could support cellular attachment and provide a suitable environment for cell proliferation and viability of anchoring cells but also can accurately control cellular alignment. Materials and Methods: CLFs were fabricated by coating a flexible biocompatible and mechanically strong collagen-based fiber by a cell-laden hydrogel layer from alginate and gelatin methacrylate (GelMA) hybrid with concentrations of 0.5-2% and 5-7.5% w/v, respectively. A motorized spool drew the core fiber through a mixed solution of alginate, GelMA, and cells and then through a solution of 2% (w/v) CaCl2 to crosslink the alginate part and form a polymeric network template (Fig. 1a). GelMA part was crosslinked by UV irradiation (365 nm, 850 mW). A dripping mechanism is designed to wash the excessive calcium from the composite fibers while keeping them in a wet environment by using tris-buffered saline (TBS). Alginate could also be removed by a calcium chelator to leave a hydrogel layer from GelMA (Fig. 1h). Fibroblast viability and metabolic activity were assessed by a standard live/dead assay and PrestoBlue assay. Cellular morphology and alignment were monitored by immunostaining of α-actin and nuclei. Results and Discussion: CLFs containing fibroblasts were created and the thickness of the gel layer could be changed from 20µm to 600µm by adjusting the drawing speed and the prepolymer concentrations (Fig. 1b-e). The encapsulated 3T3 fibroblasts showed high cellular viability (data not shown). In addition, the metabolic activity of the encapsulated cells was assessed and showed a continuous increase over 7 days of culture. Immunostaining results confirmed normal cellular morphology and alignment of encapsulated cells along fiber’s axis (Fig. 1i,j). Conclusions: A scalable technology for creating mechanically strong cell-laden fibers is reported that allows continuous fabrication of CLFS in reel-to-reel format. The fabricated fibers supported viability and proliferation and alignment of fibroblasts. National Science Foundation (EFRI-1240443); Office of Naval Research Young National Investigator Award; National Institutes of Health (HL092836, DE019024, EB012597, AR057837, DE021468, HL099073, EB008392); MIT-Italy program (ProgettoRocca); Polimi International Fellowship (PIF) Keywords: microstructure, 3D scaffold, biomimetic culture Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: General Session Oral Topic: Three-dimensional fabrication Citation: Tamayol A, Yazdi IK, Fallahi A, Nabavinia M, Avci H, Costa-Almeida R, Santiago GT, Alvarez MM, Akbari M, Annabi N and Khademhosseini A (2016). Textile tissue engineering: a path towards organ weaving. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.00108 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ali Tamayol Iman K Yazdi Afsoon Fallahi Mahboubeh Nabavinia Huseyin Avci Raquel Costa-Almeida Grissel T Santiago Mario M Alvarez Mohsen Akbari Nasim Annabi Ali Khademhosseini Google Ali Tamayol Iman K Yazdi Afsoon Fallahi Mahboubeh Nabavinia Huseyin Avci Raquel Costa-Almeida Grissel T Santiago Mario M Alvarez Mohsen Akbari Nasim Annabi Ali Khademhosseini Google Scholar Ali Tamayol Iman K Yazdi Afsoon Fallahi Mahboubeh Nabavinia Huseyin Avci Raquel Costa-Almeida Grissel T Santiago Mario M Alvarez Mohsen Akbari Nasim Annabi Ali Khademhosseini PubMed Ali Tamayol Iman K Yazdi Afsoon Fallahi Mahboubeh Nabavinia Huseyin Avci Raquel Costa-Almeida Grissel T Santiago Mario M Alvarez Mohsen Akbari Nasim Annabi Ali Khademhosseini Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.