Abstract
Japanese cedar (Cryptomeria japonica D. Don) is an important ecological and commercial species in Japan. We isolated 11 polymorphic tetranucleotide microsatellite markers by using a ligation-mediated suppression PCR technique for Japanese cedar and investigated the polymorphism of these markers in 24 individuals from natural populations. The number of alleles ranged from 6 to 20 per locus, and expected heterozygosities of the 11 loci ranged from 0.664 to 0.950. These polymorphic markers will be powerful tools for the studies of individual genotyping, population genetics, and conservation genetics.
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