Abstract
Summary Tetrahymena thermophila wild type strain B 1868 and T. thermophila disA mutant were examined for total phospholipid composition by radiolabeling whole cells with [ 32 P]orthophosphate, [ 3 H]myristic acid, and [ 3 H]acetate. Percent distribution of radioactivity into individual phospholipid classes was determined by scintillation counting or by autoradiography following chromatography of total lipid extracts on silica gel thin layer plates. A significant reduction in radiolabel incorporation into the disA mutant phosphatidylethanolamine fraction was observed in all radiolabeling experiments.
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