Tetrahydrofurane is a Component of Annona muricata Leaf will Induce Apoptosis Program in Cancer Cell because the Virus: A Proxy for Cancer Treatment

Abstract

Introduction: The compound that allegedly derived from acetogenin has a mechanism of inhibition complex of mitochondrial. Inhibition complex of mitochondrial by acetogenin will cause a decline in the production of ATP and decrease the amount of ATP will induce apoptosis. Aims: The aim of this study is whether Annona muricata (A. muricara) containing tetrahydrofurane that can induce apoptosis? Method: To detection an active isolate carried out using the method of TLC. To determine the functional group terpenoids used VLC mobile phase in chloroform-ethyl acetate then used vanillin sulfuric acid. To find out more details on cluster group were characterized by FT-IR and UV-Vis spectrophotometer. Cytotoxicity test of A. muricata leaves extract againt HeLa cells was evaluated by MTT method which saw the level of formazan absorbantion velue. Result: With vanillin sulfuric acid saw violet red color visible in light is a standard for terpenoid functional group. On the results of FT-IR spectrophotometry test indicated that the absorption of the lactone organic compound. From UV-Vis spectrophotometry test has high absorbance (wave length 222 and 230 nm) and may be a marker for the presence of tetrahydrofurane cluster compound. Of assay cytotoxicity obtained the value of R2=0.9035, namely there is a correlation in welfare between viability of HeLa cells with active isolates of A. muricata leaves. Conclusion: It is concluded that A. muricata leaves extract contain acetogenin which functional groupterpenoids, which cluster group-lactones as well as tetrahydrofurane. Cell viability of HeLa cells will progressively lower with increasing concentration of active isolates A. muricata leaves contains tetrahydrofurane