Abstract
AbstractIn this paper we analyzed the operation of K+ channels by measuring the extracellular calcium influx in response to tetraethylammonium (TEA) depolarization. We compared TEA‐induced intracellular calcium changes in fibroblasts obtained from Alzheimer Disease (AD) patients, aged (AC) and young (YC) healthy subjects. Subsequently we analyzed the effects of incubation of these fibroblasts with beta‐amyloid on calcium responses. Our results indicated that depolarization with TEA did not abolish calcium responses in any of the tested cell groups. TEA‐elicited calcium changes in AD cells were significantly greater than those observed in AC or YC cells. Responses were delayed in AD fibroblasts with respect to AC or YC cells. Incubation with beta‐amyloid did not alter the other effects: response amplitude in AD cells was still greater than in AC or YC cells; AD responses were still delayed with respect to AC and YC responses. These results support the earlier findings that calcium responses were altered in AD cells but suggest their delay rather than inhibition.
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