Tetrabromobisphenol A, terabromobisphenol S and other bromophenolic flame retardants cause cytotoxic effects and induce oxidative stress in human peripheral blood mononuclear cells (in vitro study)

Abstract

Brominated flame retardants (BFRs) are the compounds used in the industry in order to decrease flammability of various everyday products. The use of BFRs leads to migration of these substances into the environment, which results in the exposure of humans to their action. Although BFRs are widespread in human surrounding, the effect of these compounds on human body has been very poorly assessed. The purpose of this study was to evaluate cytotoxic effects as well as oxidative potential of selected bromophenolic flame retardants such as tetrabromobisphenol A (TBBPA), tetrabromobisphenol S (TBBPS), 2,4,6-tribromophenol (2,4,6-TBP) and pentabromophenol (PBP) on human peripheral blood mononuclear cells (PBMCs) that are crucial for proper functioning of the immune system. The cells were treated with the substances studied in the concentrations ranging from 0.0001 to 100 μg/mL for 1 h or 24 h. The results have shown that the compounds examined reduced PBMCs viability and ATP level as well as increased reactive oxygen species (including hydroxyl radical) formation. Moreover, the substances tested induced lipid peroxidation and caused oxidative damage to proteins in the incubated cells. It has also been noticed that the greatest changes were provoked by tetrabromobisphenol A, while the weakest by TBBPS, which is used as a substitute of TBBPA in the manufacture.