Abstract
四溴双酚A衍生物的毒理学研究亟须开展。已有研究发现四溴双酚A双（2-羟乙基醚）（tetrabromobisphenol A bis（2-hydroxyethyl ether），TBBPA-BHEE）可诱导大鼠嗜铬细胞瘤细胞（PC12）活性氧（reactive oxygen species，ROS）的生成。然而TBBPA-BHEE对PC12细胞线粒体呼吸链氧化磷酸化过程的干扰机制尚不明确，TBBPA-BHEE是否通过破坏线粒体功能干扰细胞能量代谢亟待进一步探讨。建立了基于HPLC-ESI-MS/MS分析PC12细胞内ATP、ADP、AMP及cAMP（cyclic AMP）浓度的方法，在此基础上评价了TBBPA-BHEE暴露对PC12线粒体呼吸链氧化磷酸化过程及能量代谢的影响。研究发现，TBBPA-BHEE可加速PC12线粒体呼吸链氧化磷酸化过程；TBBPA-BHEE诱导的PC12线粒体功能紊乱可引起细胞能量代谢紊乱。一方面揭示了TBBPA-BHEE对PC12潜在的毒性作用机制，另一方面也证实HPLC-ESI-MS/MS是研究细胞线粒体呼吸链氧化磷酸化及能量代谢过程的有力工具。
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