Tetanic force potentiation of mouse fast muscle is shortening speed dependent

Abstract

The activity dependent potentiation of peak isometric force associated with phosphorylation of the myosin regulatory light chain (RLC) is generally restricted to low activation frequencies. The purpose of this study was to determine if muscle shortening speed influenced the stimulus frequency domain over which concentric force potentiation was observed. To this end, mouse extensor digitorum longus (EDL) muscles (in vitro, 25 °C) were activated at a range of test frequencies (10, 25, 45, 70 or 100 Hz) during shortening ramps at 0.10, 0.30 or 0.50 of the maximal velocity of shortening (V(max)). This procedure was performed before and after a standard conditioning stimulus (CS) that elevated RLC phosphorylation from 0.08 ± 0.01 (rest) to 0.55 ± 0.01 (stimulated) moles phosphate per mol RLC, respectively (n = 9-11) (P < 0.01). When data from all test frequencies were collapsed, the CS potentiated mean concentric force at 0.10, 0.30 and 0.50 V(max) to 1.02 ± 0.03, 1.37 ± 0.03 and 1.59 ± 0.05 of unpotentiated, pre-CS values, respectively (n = 8, P < 0.05). In addition, increasing shortening speed also increased the activation frequency at which concentric force potentiation was maximal, i.e. from 10 Hz at 0.10 V(max) to 10-25 and 25-45 Hz at 0.30 and 0.50 V(max), respectively. These results indicate that both the magnitude of and activation frequency dependence for concentric force potentiation of mouse EDL muscle is shortening speed dependent. Thus, muscle shortening speed may be a critical factor determining the functional utility of the myosin RLC phosphorylation mechanism.