Abstract
The investigation included four plum (Prunus domestica L.) hybrids, which were selected on the base of visual inspection in natural infection background and serological analyses. Two of the hybrids were obtained from hybridization of ‘Pasific’ × ‘Serdika’ (21-3 and 21-47) and another two were the result from crossing of ‘Pasific’ × ‘Stanley’ (21-55 and 21-67). The tests for resistance to Plum pox virus (PPV) combined biological indexing and field trial. In the biological indexing the hybrids were inoculated artificially by chip budding under greenhouse conditions, with isolates representing PPV-M, PPV-D and PPV-Rec strains of the virus. Up to now the data of the biological indexing showed that the hybrid 21-47 did not manifest symptoms of PPV and ELISA results were negative. The other three hybrids were infected by PPV isolates used. Simultaneously, the studied hybrids were propagated and planted under natural PPV infection pressure, without chemical protection against aphids. The results of the field trial after 5 years growing showed that hybrid 21-47 remained symptomless and reacted negatively with an antiserum to PPV, while in the other three hybrids 21-3, 21-55 and 21-67, the virus was detected respectively in 7.7, 23.5 and 42.1% of tested plants. At that stage of the study on the base of the summarized data, it could be accepted that the hybrid 21-47 possesses a high level of resistance to PPV.
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