Tests for recombinagens in fungi

Abstract

Three types of mitotic recombination can be studied in Aspergillus nidulans and Saccharomyces cerevisiae: (1) The classical type of reciprocal mitotic crossing-over which can be detected when it occurs between non-sister chromatids at the four-strand stage followed by co-segregation of a crossing-over and a non-crossing-over chromatid in the subsequent mitotic division. Consequently, mitotic crossing-over reflects cellular responses to primary genetic damage in the G 2 phase of the cell cycle. (2) Mitotic gene conversion is a unidirectional event of a localized transfer of genetic information between non-sister chromatids which in yeast can extend to segments of up to 18 cM and even beyond 22 cM in Aspergillus nidulans. Mitotic gene conversion can also occur between unreplicated chromatids and lead to the expression of the newly created genotype without any need for a subsequent mitotic cell division. It reflects a cellular response in G 1. (3) Mitotic sister-strand gene conversion can be studied in a recently constructed strain with the same technical ease as classical non-sister chromatid gene conversion. It can be induced by chemicals which do not induce mutation in the Salmonella system and non-sister chromatid gene conversion. Mitotic segregation in Saccharomyces cerevisiae results almost exclusively from crossing-over and gene conversion whereas mitotic chromosomal malsegregation contributes only very little. In contrast to this, in Aspergillus nidulans, both processes contribute considerably so that mitotic segregants always have to be tested for their mechanistic origin.