Abstract

Incubations of minces of rat testes with pregnenolone-[4- 14C]-[17α- 3H] yielded testosterone containing both labels. The oxidation of this metabolite to androstenedione with chromic acid did not result in the loss of tritium. These results argue against conversion of pregnenolone or progesterone to testosterone acetate by direct oxygen insertion, as in the nonbiological Baeyer-Villiger reaction.

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