Testosterone administration enhances the expectation and perception of painful and non-painful somatosensory stimuli

Abstract

The influence of testosterone on pain perception remains inconsistent in the literature. This randomized, placebo-controlled, double-blind, crossover study investigated the effect of testosterone administration on perception and expectation of electrocutaneous stimulus. Thirty healthy male participants received a single dose of testosterone in one session and a placebo in the other session. For each session, they completed a pain-rating task in which a predictability cue was inserted before a painful or non-painful electocutaneous stimulus delivery, while neural activity was simultaneously recorded by a 64-channel electroencephalographic (EEG) system. Expected and perceived pain ratings, as well as event-related potentials (ERPs) to electocutaneous stimuli and prestimulus EEG oscillatory activities while expecting upcoming electocutaneous stimuli were comprehensively compared between testosterone and placebo sessions. Compared with the placebo session, participants in the testosterone session reported greater pain rating and exhibited greater amplitude of N1 component on ERPs when perceiving both painful and non-painful electrocutaneous stimuli. Mediation analysis revealed that testosterone enhanced the pain-intensity ratings via the N1 response to the electrocutaneous stimulus. Upon viewing the predictability cues after testosterone administration, expected pain intensity increased and spontaneous low-frequency α-oscillation power in the frontal region decreased. These results provide evidence that testosterone enhanced perception and expectation of somatosensory events, and that this was a general effect rather than pain-specific. A plausible explanation for these findings is that testosterone acts to increase vigilance and sustained attention levels, as evidenced by the decreased α-oscillation power. Thus, our findings support a causal role for testosterone in heightening the biological salience of incoming somatosensory information.