Testing the Effect of Some Materials to Control Pythium Aphanidermatum Isolated from Cucumber

Abstract

he main objective of this study was to identify the effectiveness of household materials that are used to sterilize vegetables from the fungus Pythium aphanidermatum (P. aphanidermatum). Samples were taken from the damaged cucumber in the local markets of Babil Governorate, Iraq. These samples were kept in sterile cool containers until they reached the Microbiology lab. of the Department of Community Health, Technical Institute of Babylon. The samples were cultured laboratory in the Sabouraud Dextrose Agar (SDA), then a microscopic examination was carried out to identify the fungus using the lacto phenol cotton blue dye. Use in this study most common household materials to sterilize vegetables before keeping them in the refrigerator or before eating are Potassium Permanganate (KMnO4), Sodium Hypochloride (NaOCl) and table salt (NaCl). Five concentrations were used for each sterile material, and five replicates of culture dishes were used for each concentration (A mixture of sterile material with the SDA) after which the fungus was cultured. By using the minimum inhibitory concentration assay, the radial growth inhibition assay and the microscopic fungal examination, the efficacy of sterilizers was identified. The final results showed the presence of antifungal efficacy for all sterilizers used, with different concentrations. This study concluded that these materials can be used by housewives to sterilize vegetables before keeping them in the refrigerator or eating them. How to Cite: Ibtisam Mohammed Hussein, Abbas Razzaq Abed, 2023. "Testing the Effect of Some Materials to Control Pythium Aphanidermatum Isolated from Cucumber." Journal of Agriculture and Crops, vol. 9, pp. 266-272.