Abstract
McAndrew, M., Graham, S., Hartmann, C., and Clayton, C. 1998. Testing promoter activity in the trypanosome genome: Isolation of a metacyclic-type VSG promoter, and unexpected insights into RNA polymerase II transcription.Experimental Parasitology90, 65–76. In trypanosomes, most genes are arranged in polycistronic transcription units. Individual mRNAs are generated by 5′-transsplicing and 3′ polyadenylation. Remarkably, no regulation of RNA polymerase II transcription has been detected although many RNAs are differentially expressed during kinetoplastid life cycles. Demonstration of specific class II promoters is complicated by the difficulty in distinguishing between genuine promoter activity and stimulation oftranssplicing. Using vectors that were designed to allow the detection of low promoter activities in a transcriptionally silent chromosomal context, we isolated a novel trypanosome RNA polymerase I promoter. We were however unable to detect class II promoter activity in any tested DNA fragment. We also integrated genes which were preceded by a T3 promoter into the genome of cells expressing bacteriophage T3 polymerase: surprisingly, transcription was α-amanitin sensitive. One possible interpretation of these results is that in trypanosomes, RNA polymerase II initiation is favored by genomic accessibility and double-strand melting.
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