Testing Phylogeographic Hypotheses in Mepraia (Hemiptera: Reduviidae) Suggests a Complex Spatio-Temporal Colonization in the Coastal Atacama Desert

Abstract

Simple SummaryMepraia is a blood-sucking bug endemic to Chile and a vector of the parasite that causes Chagas disease. Different colonization routes have been suggested for this bug; therefore, we tested different colonization routes using DNA sequences and bioinformatics approaches to select the most probable route. Our results suggest that, after the split of Triatoma, Mepraia divided into two main groups ~2.1 Mya. The northern group would have speciated between 1.7–1.4 Mya, giving rise to M. parapatrica, M. gajardoi and to a new, still undescribed lineage (Mepraia sp.). The southern group formed M. spinolai ~1.68 Mya. We suggest that Mepraia originated from the north-central Andes due to the last Andes uplift and hyperaridity. The hyperarid cycle would have separated the southern and northern groups. Then, within the northern group, colonization would have occurred from the centre to the north and south through corridors influenced by Pleistocene climatic changes. The habitat colonized by the southern clade led to only one species (M. spinolai). Fluctuations in climatic changes probably influenced speciation strongly in this kissing bug in the Atacama Desert.Mepraia is a genus (Triatominae) endemic to Chile and a vector of Trypanosoma cruzi. Alternative phylogeographic hypotheses have been suggested for Mepraia. We tested different colonization routes hypothesized using mitochondrial sequences and phylogeographic approaches to select the best-supported hypothesis. Our results suggest that, after the split from the sister genus Triatoma at ~4.3 Mya, Mepraia formed two main clades at ~2.1 Mya. The northern clade diverged from Mepraia sp. ~1.7 Mya, giving rise to M. parapatrica and M. gajardoi about ~1.4 Mya. The southern clade originated M. spinolai ~1.68 Mya. We suggest that Mepraia had an origin in the north-central Andes along with orogenic processes, reinforced by hyperaridity during the Pliocene. The hyperarid cycle would have separated the southern and northern clades. Then, in the northern clade, dispersal occurred north and south from the centre through corridors during the Pleistocene Climatic Oscillations. Climate changes may have induced a major speciation process in the Atacama Desert, while the more homogeneous habitat colonized by the southern clade led to only one, but structured, species.