Testing continuity in a Michigan (USA) river using the organic biomass of adult Ephemeroptera, Plecoptera, and Trichoptera

Abstract

While there is substantial support for the river continuum concept (RCC) using aquatic macroinvertebrates, nearly all of this support has come from studies using benthic specimen counts or relative biomass as the basis for comparing sites. We instead assessed the RCC using the ash-free dry mass (AFDM) of winged adult Ephemeroptera, Plecoptera, and Trichoptera (EPT) specimens. Insects were sampled at 13 sites along a 100 km 2nd–4th order reach of the Pine River in northern Lower Michigan using ultraviolet lights. Specimens were identified to the genus level and the AFDM of each functional feeding group (FFG) was calculated. Forty-two environmental and river morphological variables were tested for their ability to predict differences in EPT FFG biomass. A determined environmental gradient relating to increasing river size predicted over 80% of FFG biomass differences. Biomass of each individual FFG increased along the determined gradient, except for scrapers which decreased at the most downstream sites. The number of EPT genera also increased along the gradient. Our results were similar to those of previous studies, except that shredders nearly doubled in importance compared to RCC predictions, suggesting a greater ecological impact of shredders on forested streams than is typically reported.