Abstract
Large numbers of endogenous A-type and C-type virus particles are produced by many hybridoma cell lines, yet the effects of virus production on the metabolism of the cell are poorly understood. Previous studies using an advanced computer model of cell growth in batch culture indicated that virus production may affect both cell numbers and antibody yield. We have continued to look for viral effects predicted by the simulations experimentally. Virus production by a cell line that was sufficiently well characterised for simulation studies was altered chemically and hormonally. The number of virus particles was measured to determine if changes in particle numbers correlated with changes in cell growth as predicted by the computer simulation. The various techniques available for controlling virus particle expression and detecting these changes were evaluated.
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