Abstract
The techniques employed for bioconversion of cellulosic biomass to simple sugars by cellulases have a great industrial brunt. Cold-adapted microorganisms are potential resource of cold-active carboxymethyl cellulases secluded from the cold regions. In this study, Trichophyton terrestre -a rare fungal species in Indian soils, isolated from the rhizosphere of Juglans regia L. during winter season was subjugated for the production and commotion of an enzyme carboxymethyl cellulase in carboxymethyl cellulose solution by DNS method at an ample range of temperatures, using Lineweaver-Burk plot which offers a practical graphical method for the analysis of Michealis-Menten equation, to establish the imperative terms in enzyme kinetics as K m and V max . The enzyme kinetic parameters like maximum activity (V max ), K m and turnover number were recorded at varying concentrations of CMC and different temperatures (4°C and 50°C). The enzyme was found to be tolerant and stable at two varying temperatures which enables this fungal species to survive in the extreme environmental conditions of northern India. Such property of carboxymethyl cellulase enzyme has extensive range of applications and the potential to open new application fields in the areas of industrial processes.
Highlights
Human kind has benefited enormously from the community
Trichophyton terrestre-a rare fungal species in Indian soils, isolated from the rhizosphere of Juglans regia L. during winter season was subjugated for the production and commotion of an enzyme carboxymethyl cellulase in carboxymethyl cellulose solution by Dinitrosalicylic Acid (DNS) method at an ample range of temperatures, using Lineweaver-Burk plot which offers a practical graphical method for the analysis of Michealis-Menten equation, to establish the imperative terms in enzyme kinetics as Km and Vmax
Among an array of fungal strains isolated during rainy, spring and winter seasons from the rhizosphere of Juglans regia L., geophilic fungus Trichophyton terrestre was isolated only during the winter season and screened for the production and activity of the enzyme carboxymethyl cellulase
Summary
Human kind has benefited enormously from the community They produce the novel and stable study of microbes since their discovery in the 17th enzymes which function under extreme conditions century. Such benefits have come from the comparable to those prevailing in various industrial study of just a miniscule fraction of the millions of the processes. The tendency of Cellulose is the principal structural polysaccharide surviving in such environmental conditions comes more in plants and the most abundant biomass on earth It is or less directly from the enzyme stability which composed of β-1, 4-linked glucose units which enhances the survival rates of these microbes in harsh contains both highly crystalline and amorphous conditions. Cellulase enzymes indicate the potentiality of successful conversions of waste cellulosics into foods for our burgeoning population, the enzyme exploitation subject demands the intense research even at molecular level (Demain and Solomon, 1985)
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