Testing a New Anti-Zebra Mussel Coating with a Multi-plate Sampler: Confounding Factors and other Fuzzy Features

Abstract

In situ experiments were conducted to assess the use of multi-plate samplers for antifouling experiments and to test the antifouling effectiveness of a chitin-based coating against zebra mussels (Dreissena polymorpha). A series of multi-plate samplers consisting of three parallel plates coated with chitin, and untreated control samplers, were submerged horizontally or vertically in a marina of the St Lawrence River for 3 1/2 months (July-October) in 1998 and 1999. Mussels attached to the chitin-treated substrates were on average 2.75 times more abundant than on the control samplers, indicating that chitin is not effective as an antifouling agent against zebra mussels. The abundance, size distribution and spatial dispersion of mussels on the plates varied both between plates and between top vs bottom sides of plates in horizontal collectors, but not in vertical samplers. The three plates composing each multi-plate horizontal sampler do not represent true replicates for statistical analysis. The bottom side of plates exhibited the least variability and might therefore serve as the experimental unit. Substrate heterogeneity and plate orientation were identified as confounding factors to be controlled for in future experiments. Sunlight exposure and colonization by sponges strongly influenced zebra mussel abundance and should be considered when performing in situ experiments. Because of the influence of uncontrolled factors, it is recommended that in situ pilot studies be conducted to statistically test the effectiveness of antifouling products once the threshold level of the desired effectiveness is defined.