Abstract
Testicular tissue grafting and male germ cell transplantation are techniques that offer unprecedented opportunities to study testicular function and development. While testicular tissue grafting allows recapitulation of testis development and spermatogenesis from immature males of different mammalian species in recipient mice, germ cell transplantation results in donor-derived spermatogenesis in recipient testes.Testicular tissue grafting results in spermatogenesis from a wide variety of large animal donor species and is therefore an attractive way to study testis development and spermatogenesis and preserve fertility of immature males. Germ cell transplantation represents a functional reconstitution assay for identification of spermatogonial stem cells (SSCs) in a given donor cell population and serves as a valuable tool to study stem cell biology and spermatogenesis. In this chapter we provide detailed methodology to successfully perform both techniques.
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