Testicular sperm correlates with a similar rate of euploid embryo development compared to cycles using ejaculated sperm

Abstract

To evaluate the percentage of euploid embryo development in patients with obstructive and non-obstructive azoospermia. Retrospective data analysis. From March 2013 to April 2016, 36 azoospermic men underwent 45 cycles of ICSI-PGS cycles with blastocyst trophoectoderm biopsy for comprehensive chromosomal screening using high density oligonucleotide microarray comparative genomic hybridization. 30 cycles used testicular sperm from obstructive azoospermia men with prior vasectomy, 9 cycles were non-obstructive, and 6 cycles were from azoospermic men with unknown causes. The results were compared with 621 In-Vitro Fertilization (IVF) patients that used ejaculated sperm during the same time period. 3,475 blastocysts were generated and underwent trophoectoderm biopsy and PGS. Tabled 1Age group# ICSI Cycle# MII eggs ICSI’d#2PN(Fert rate)Blastocyst # (rate)Euploid #(rate)P value<37TESE38446341 (76.5%)206 (60.4%)133/163 (81.6%)P=0.6<37EJ40143723096 (70.8%)3271/5361 (61.0%)1911/2491 (76.7%)38-42TESE75448 (88.5%)19 (39.6%)10/19 (52.5%)P=0.438-42EJ2201044885/1044 (84.8%)771/1537 (50.2%)306/802 (38.2%) Open table in a new tab Our data demonstrate that testicular sperm from azoospermic patients have comparable fertilization, blastocyst formation and euploid rates compared to cycles using ejaculated sperm. These comparable rates of fertilization, blastocyst formation and rates of euploid embryos with the use of testicular sperm requires further study but may be due in part to female partners with more favorable fertility potential.