Testate Amoebae as Sea-level Indicators in Northwestern Norway: Developments in Sample Preparation and Analysis

Abstract

Proxy based sea-level reconstructions are an important tool for defining past patterns of sea-level change and salt-marsh testate amoebae are a newly emerging proxy with high potential as sea-level indicators. This study develops existing analytical techniques con- cerned with the preparation and counting of testate amoebae for sea-level studies and demonstrates the predictive power of this group of micro-organisms. Two salt marshes in northwestern Norway were sampled for testate amoebae and multiple sub-samples were prepared using different procedures and count totals. Analytical efficiency can be improved upon by using a mild alkali, chemical disaggregant (5% KOH) to break up fibrous, salt-marsh peat and concentrate tests prior to counting. A count total of 100 individuals, rather than 150, can be used to make time gains with little or no loss of taxon information. The Norwegian salt-marsh testate amoebae showed strong zonation relative to tidal elevation. Key indicator taxa from the high marsh included Centropyxis cassis type, Cyclopyxis arcelloides type and Eugly- pha spp. Those from the low marsh included Difflugia pristis type and a distinctive morphotype of Centropyxis platystoma type. Combined, the two surface data sets from Norway were capable of predicting marsh surface elevations to within ± 0.09 m.