Abstract
Rapid detection of bacterial contamination is an essential task in numerous medical and technical processes and one of the most rapidly developing areas of nano-based analytics. Here, we present a simple-to-use and special-equipment-free test-system for bacteria detection based on magnetite nanoparticle arrays. The system is based on peroxide oxidation of chromogenic substrate catalyzed by magnetite nanoparticles, and the process undergoes computer-aided visual analysis. The nanoparticles used had a pristine surface free of adsorbed molecules and demonstrated high catalytic activities up to 6585 U/mg. The catalytic process showed the Michaelis–Menten kinetic with Km valued 1.22 mmol/L and Vmax of 4.39 µmol/s. The nanoparticles synthesized were used for the creation of inkjet printing inks and the design of sensor arrays by soft lithography. The printed sensors require no special equipment for data reading and showed a linear response for the detection of model bacteria in the range of 104–108 colony-forming units (CFU) per milliliter with the detection limit of 3.2 × 103 CFU/mL.
Highlights
Pathogenic bacteria cause many globally essential diseases
Ag and Au nanoparticles can be used for surface-enhanced Raman scattering (SERS) [9], carbon nanotubes for potentiometry [10], Ncaanromboatnerinalas n20o2t0u, b10e,s31f3or potentiometry [10], CdSe [11], and CdS [12] quantum dots in the fluores2coefn1c1e microscopy and other materials and methods have found their application in bacteria sensing as well
The stable hydrosol of magnetite nanoparticles was obtained by the US-assisted coprecipitation procedure using a mixture of ferric and ferrous chlorides and water–ammonia solution as a base [29]
Summary
Pathogenic bacteria cause many globally essential diseases. Even if the number of dangerous pathogens is negligible in a sample, it can lead to severe consequences [1]. Rapid detection of hazardous bacteria in air, water, and food can improve the quality of clinical diagnosis and reduce the rate of disease transmittance. In its turn, reducing detection time and increasing overall sensitivity may be developed by the application of molecularly-amplified (eq polymerase chain reaction) and enzyme-amplified (eq enzyme-linked immunosorbent assay) methods. These techniques are expensive and require specialized laboratory facilities [5]. The ensuing solution of magnetite nanoparticles exhibited the magnetic liquid properties, when a magnetic field was applied, the particles did not separate from the water. The mass fraction of magnetite in the resulting colloid solution was 2% wt
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