TEST OF EXUDATE pH IN RICE SEEDS

Mariely De Abreu Dos Santos,Josué Bispo Da Silva,Izabela Cristina De Oliveira,Ana Carina Da Silva Candido,Charline Zaratin Alves,Graziely Alves Nogueira

doi:10.1590/1983-21252019v32n412rc

Mariely De Abreu Dos Santos, Josué Bispo Da Silva + Show 4 more

Open Access

https://doi.org/10.1590/1983-21252019v32n412rc

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Journal: Revista Caatinga	Publication Date: Dec 1, 2019
Citations: 2	License type: CC BY 4.0

Affiliation: Universidade Federal de Mato Grosso do Sul

Abstract

ABSTRACT The objective of this study was to evaluate the efficiency of a pH test of the exudate (with and without tegument) as an indicator of the physiological quality of rice seeds. The experimental design was completely randomized, with seven lots and four replications. The seven lots were assessed for water content and initial physiological quality by means of germination, first germination count, emergence, emergence speed index and tetrazolium tests. The pH test of the exudate was conducted with 100 seeds of each treatment, with tegument and without tegument. The seeds were individually soaked in 2.0 mL distilled water for different periods (20, 40, and 60 minutes) and three temperatures (20, 25, and 30 °C). The results were submitted to the Scott Knott test at 5% probability. The pH test of the exudate is a promising method to evaluate the physiological quality of rice seeds by detecting differences in vigor between marketable lots. This test should be conducted with rice seed without tegument, at 25 °C, with a soak time of 60 minutes.

Highlights

The evaluation of the physiological quality of rice (Oryza sativa L.) through the standard germination test is somewhat slow, since the results require between 5 and 14 days, which is a long period for decision making on seed lots
Vigor tests aim to identify significant variations in the physiological quality of lots considered suitable for commercialization
The results obtained in the tetrazolium test indicated lots 1, 2 and 4 as being of high vigor, while lots 3, 5 and 7 were classified as being of intermediate vigor and lot 6 was classified as being of low vigor (Table 1)

Summary

Introduction

The evaluation of the physiological quality of rice (Oryza sativa L.) through the standard germination test is somewhat slow, since the results require between 5 and 14 days, which is a long period for decision making on seed lots. Vigor tests aim to identify significant variations in the physiological quality of lots considered suitable for commercialization. These tests help in classifying seeds based on vigor levels, especially seedling emergence ratio in the field (MARCOS FILHO, 2015). Vigor tests that provide results quickly must be developed, since the evaluation of seed quality depends on the efficiency of the procedures involved (LOPES; SILVA; VIEIRA, 2013). More rapid tests allow agility in decision-making, are usable on a broader scale, and reduce the risks and costs involved in operations, such as harvesting, processing, storage, and marketing (HILST et al, 2012)

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