Abstract
A cell exhibits microtubule-motor-dependent spatiotemporal organization of its subcellular structures. Previously, we observed self-organized asters in the ooplasm enclosed within hexagon dominant polygonal domains similar to the cell packing in epithelial tissues. However, an active, non-crystalline and crowded cell often deviates from an ideal packing behaviour. Here, we discuss the image analysis pipeline and the geometric method to characterize the features of the spatial organization. The algorithm automatically (or user-defined) detects the aster centers that serve as seed points for Voronoi tessellations.
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