Abstract
The possibility of designing a model of sensor to antioxidants based on thiacalix[4]arene monolayers with immobilized cytochrome c was shown. The molecular surface area S0 of thiacalix[4]arene in the monolayers and the surface pressure coefficient −dπ/dS (elasticity) reflect changes in the redox state of cytochrome c in the presence of dihydroquercetin and ascorbic acid in the aqueous subphase. The absorption spectra in the visible and UV ranges of solutions of the subphase and transferred thiacalix[4]arene monolayers with immobilized cytochrome c confirm the oxidation of the antioxidants to quinones and formation of the reduced form of cytochrome c.
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