Terrestrial anaerobic digestate composite for fertilization of oligotrophic coastal seas

Abstract

To promote primary production in oligotrophic seas, we developed a fertilization composite consisting of blast furnace cement and anaerobic digestate with a high nutrient content derived from biogas power plant byproducts. In this study, we investigated the dissolution behavior of nutrients from the fertilization composite and evaluated the effects of the fertilization composite on the growth of marine primary producers. Batch experiments and tank experiments to simulate oligotrophic coastal seas revealed that the nutrients dissolved from the fertilization composite were taken up by marine microalgae and seaweed. The fertilization composite promoted the growth of both planktonic and benthic micro algae. The total amounts of chlorophyll a in the fertilization composite tank increased by 1.4 times compared to control (p < 0.01). The flow of dissolved inorganic nitrogen uptake by marine microalgae increased 3–5 times when the fertilization composite was applied. In Wakame seaweed culture experiments, the nitrogen contents of Wakame from the fertilization composite tank were 1.2 times higher than those cultured in the control tank (p < 0.01). As a result, Wakame leaves cultured in the fertilization composite tank were 1.4 times longer than those cultured in the control tank. Approximately 44% of dissolved inorganic nitrogen from the fertilization composite was taken up by the seaweed. Hence, the fertilization composite was demonstrated to enhance the growth of marine microalgae and seaweed, which are primary producers in marine ecosystems. The fertilization composite proposed in this study can create novel nutrient mass flow by connecting terrigenous anaerobic digestate from biogas power plants to oligotrophic seas and thus stimulate the recovery of fishery production.