Terpenoids with cytotoxic activity from the branches and leaves of Pyrus pashia

Abstract

Twenty terpenoids, including a new triterpenoid (1) and a new monoterpenoid (20), were isolated from the branches and leaves of Pyrus pashia. The structures of two new compounds were determined to be 2α, 3β, 27-trihydroxyolean-12-en-28-oic acid (1) and (4α)-3-(5,5-dimethyltetrahydrofuranyl)-1-buten-3-ol 3-O-β-d-glucopyranoside (20) on the basis of spectroscopic analysis (IR, HRESIMS, 1D and 2D NMR) and chemical method. Some of the isolated compounds were evaluated for their cytotoxic activity against a panel of human cancer cell lines by MTT assay, using cisplatin as a positive control. Compound 14 exhibited cytotoxic activities against A549 (IC50=19.18±4.26μM), Hela (IC50=12.56±3.89μM), SGC7901 (IC50=10.48±1.95μM) and NHI-1975 (IC50=7.38±2.31μM) cell lines as well as compound 12 displayed cytotoxic activities against A549 (IC50=14.71±1.47μM) and Hela (IC50=12.22±1.88μM) cell lines.