Abstract
Serratia plymuthica 4Rx13 emits the unique hydrocarbon sodorifen (C16H26) in which every carbon of the bicyclo[3.2.1]octane skeleton carries a methyl unit. Biosynthesis of this homosesquiterpene depends on a gene cluster comprising four genes, an isopentenyl pyrophosphate (PP) isomerase, a deoxyxylulose-5-phosphate synthase, a methyltransferase (MT), and a terpene synthase. The latter two enzymes are required and sufficient for the noncanonical biosynthesis of sodorifen.
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