Abstract
To investigate the possible protective role of the bioflavonoid ternatin (TTN) when administered before induction of ischemia/reperfusion injury in rat testis. Thirty-six Wistar rats were randomly assigned to 3 groups (n=12), divided in 2 subgroups (n=6). Saline 2.0ml (G-1), dimethylsulfoxide (DMSO) 3% solution (G-2) or TTN 12 mg/kg/dose (G-3) was administered ip. to all rats, respectively, 21, 12 and 1 hour before torsion. Anesthetized rats were subjected to ischemia (3 hours) induced by 720º torsion of the spermatic cord. Right testis and arterial blood samples were collected at the end of ischemia (T-0), and 3 hours later (T-3) for assessment of testis malonaldehyde (MDA), reduced glutathione (GSH), and plasma total antioxidant power (TAP). MDA decreased significantly (p<0,001) in G-2 and G-3 in T-0 and T-3 timepoints. Additional decrease in MDA was seen in G-3 after 3 hours of reperfusion (T-3). GSH increased significantly in G-2 (p<0.001) and G-3 (p<0.05) at the end the ischemia (T-0). A significant increase in GSH was seen 3 hours after testis detorsion (T-3) in G-2 rats. TAP values remained unchanged. The data provides in vivo evidence of the antiperoxidative and antioxidative properties of TTN in torted rat testis.
Highlights
Introduction were likewise storedFlavonoids are claimed to have protective effects against free radicals induced lipid peroxidation of living cell membranes[1,2]
Events occurring during testicular torsion/detorsion (T/D) are representative of an ischemia/reperfusion (I/R) type of injury observed in other organs[7]
The results of the present study support the view that ternatin can exert a protective effect against testis tissue lipid peroxidation injury and oxidative stress caused by ischemia/ reperfusion in rats subjected to T/D of the spermatic cord. 2000;59(10)
Summary
Flavonoids are claimed to have protective effects against free radicals induced lipid peroxidation of living cell membranes[1,2]. Their protective effects may be due to their ability to scavenge reactive oxygen species[3], free hydroxyl and peroxyl radicals[4], or to chelate iron ions[5], which are involved in the initiation of free radical reactions. Ternatin (TTN), a tetramoxyflavone from Egletes viscosa L., is known to have protective effects against hepatic oxidative damage induced by aflatoxin B1, a metabolite of Aspergillus flavus and a potent hepatotoxic[6]. Experimental unilateral spermatic cord torsion has shown to induce testicular biochemical changes indicative of lipid peroxidation[8,9,10,11]
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