Abstract
Chlorophyll (Chl) is of great potential for food development as an important colorant and functional ingredient. However, the weak stability of it severely restricts its application. This study shows a mechanism for enhancing Chl stability through ternary synergistic aggregation of Chl/SPI. Under heat treatment, the improvement rate of Chl/Pr synergistic aggregation on Chl retention was 200.34% of the low Chl concentration Chl/Pr sample and 117.10% of the high-concentration Chl sample. Spectral analysis and inverted fluorescence microscope observation confirmed the existence of Chl/Pr synergistic aggregation. The Pr self-aggregate provided an ideal hydrophobic cavity for the self-aggregation of Chl, enhancing the stability of Chl. In the synergistic aggregation, Chl self-aggregates and Pr self-aggregates competed for binding sites with Chl/Pr aggregates through hydrophobic interaction, forming a dynamic equilibrium. It is hoped that this study can perfect the mechanism of Chl/Pr interaction and provide new ideas for improving natural pigments stability.
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