Abstract
Incubation of CHO cells with 21 μM [ 3H]9-β-D-xylofuranosyladenine for 2 hr resulted in the intracellular accumulation of 9-β-D-xylofuranosyladenine 5′-triphosphate and the incorporation of radioactivity into HC1O 4 -insoluble material. After incubation of the HC1O 4 -insoluble material in 0.3 N KOH for 16 hr, the radioactivity associated with the nucleoside fraction coeluted with authentic 9-β-D-xylofuranosyladenine by high-pressure liquid chromatography and was resistant to oxidation with NaIO 4. Upon incubation with adenosine deaminase (E.C. 3.5.4.4.), the radioactivity coeluted with xylosylhypoxanthine. The data suggest that termination of RNA chains by xylosyl nucleotides may be a mechanism for producing the toxicity of 9-β-D-xylofuranosyladenine.
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