Terminalia catappa fruit extract effect on biochemical parameters of the brain like oxidative stress, amyloid beta and Acetylcholinesterase

Abstract

AbstractBackgroundThe present research deals with the assessment of Anti‐Alzheimer affect Terminalia catappa fruit extracts (TCFE) in Alzheimer diseased (AD) rat and also propose its probable mechanism of action.MethodAlzheimer disease was induced by administering streptozotocin i.e. STZ (3 mg/kg) by Intracerebroventricular injection (i.c.v. injection) day 1st and 3rd day, after surgery. Stereotaxic apparatus was used for surgery. Anesthetized rats were used for surgery. Streptozotocin induced Alzheimer diseased rats were treated with hydroalcoholic extract of TCFE (100 and 200 mg/kg, p.o.) for 14 days. Effects of TCFE in Alzheimer diseased rats were assessed for biochemical parameter in the brain tissue oxidative stress parameters (SOD, CAT, GSH and TBARS), amyloid β peptide (Aβ) and acetylcholinesterase (AchE)ResultTCFE significantly (p<0.01) decreases the Aβ1‐40, Aβ1‐42 peptides. Acetylcholinesterase level in the rats brain tissue reduced compared to Alzheimer diseased rats. TCFE significantly decreases the oxidative stress level in Alzheimer diseased rats.ConclusionEffect of TCFE on superoxide dismutase, catalase, reduced glutathione and Thiobarbituaric acid reactive species in the brain tissue of STZ treated AD rats were shown in Table 1.TCFE significantly increased level of antioxidants in brain tissues ((P< 0.01)). β amyloid level of Aβ1‐40 and Aβ1‐42 in the brain tissue decreased significantly (p<0.01) in TCFE treated group compared to AD rats (Negative control group)(Table 2) STZ administration produced significant elevation in brain AchE activity compared to control group of rats. However, TCFE significantly inhibited (P< 0.01) of AchE activity in brain tissue as compared with the negative control group of rats.