Abstract
The terminal oxidase of Hamlin orange was perturbed with the inhibitors potassium cyanide (KCN) and salicylhydroxamic acid (SHAM) to determine functionality of the pathway during ripening. Mitochondrial fractions were prepared from juice vesicles of Hamlin oranges harvested over the maturation season, September to January. The NADH oxidase became more sensitive to KCN and SHAM as the fruit matured. The KCN-insensitive oxidase of mature fruit inhibited by SHAM accounted for about 30% of the total. Oxidation of malate by preparations from December and January fruit was inhibited about 90% by KCN plus SHAM. The fraction of the alternative path which is in actual use by the mitochondria in maturing fruit varied from 0.4 to 0.5 with malate and 0.2 to 0.3 with NADH as substrates.
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