Abstract
Selective tritium labelling method for the determination of C-terminal amino acids was applied successfully to proteins whose carboxyl residue is not available to carboxypeptidases A & B or to hydrazinolysis methods. The carboxyl and amino residues of prothrombin were found to be glutamic acid (or glutamine) and alanine, respectively. The C-terminal amino acid of prothrombin did not change by DEAE-cellulose chromatography. Prothrombin obtained from dicoumarol-treated steers also had NH 2-terminal alanine and C-terminal glutamic acid (or glutamine). The similar characteristics of the two preparations (normal and dicoumarol-treated) suggest that the polypeptide chain of the abnormal molecules was not significantly different from that of normal.
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