Abstract
Fluorescence analysis is a simple and a highly sensitive method for detection of small amounts of biologically active substances. In this study, a complexation of terbium(III) chelates with 1,10-phenanthroline and ascorbic acid (AA) and luminescent properties of complexes were investigated. The influence of pH and solubilization of complexes by micellar solutions of nonionic, cationic, and anionic surfactants on fluorescence was studied. The quenching effect of terbium ion fluorescence was detected upon an introduction of ascorbic acid. The quenching effect of the complex with mixed ligands Tb(1,10-phenanthroline)-AA allows for the detection of ascorbic acid with the limit of 7.4 × 10−5 mol·L−1.
Highlights
The fluorescence analysis is a method that utilizes sensitized luminescence of lanthanide complexes and is a highly sensitive technique broadly used in analytical chemistry and scientific research in biology and biomedicine [1,2]
The energy of the triplet level of phenanthroline C12 H8 N2 (Phen) is 22,075 cm–1 ; this value is higher than the energy of the resonance level of the terbium ion at
This makes possible the transfer of excitation energy from the organic ligand molecule to the terbium ion, which contributes to sensitized fluorescence
Summary
The fluorescence analysis is a method that utilizes sensitized luminescence of lanthanide complexes and is a highly sensitive technique broadly used in analytical chemistry and scientific research in biology and biomedicine [1,2]. This method is suitable for the detection of compounds that are capable of forming complexes with lanthanide ions Ln(III). In such complexes, an analyte acts as either a sensitizer or a quencher.
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