Abstract

SUMMARYA tentative identification of some sterols present in mycelia and conidia of Fusarium roseum was made by gas liquid chromatography. Sterols identified were ergosterol, 22-dihydroergosterol, and 7-dehydroclionasterol. On ergosterol amended medium (10 mg/liter) conidial production was reduced and the percentage of mature macroconidia was increased from SO to 70%. After 44 days in distilled water at 4 C, germination of conidia produced on ergosterol amended medium was 16%, whereas, germination of conidia produced on medium without added sterols was 6%. Production of mycelium or conidia was not affected by addition of 0, 5,000, 10,000, or 50,000 ppm DMSO to the medium. However, after 70 days storage in distilled H2O at 4 C, approximately 34% of conidia produced on medium with 0, 5,000, 10,000, and 25,000 ppm DMSO germinated, whereas none of the conidia produced on medium with 50,000 ppm DMSO germinated.

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