Tensile characteristics of collenchyma cell walls at different calcium contents

Abstract

Collenchyma fibres from celery (Apium graveolens L.) were extracted with detergent and phenol‐acetic acid‐water to leave the intact cell walls, free from active enzymes. Under a small, constant stress the cell wall fibres showed elastic and plastic extension and viscoelastic deformation, but viscous flow was observed only at high stresses close to the breaking stress. After complete removal of calcium ions with cyclohex‐anediamine tetraacetic acid (CDTA) and incubation for 18 h, comparable levels of these extensibility components were observed at much lower stresses. However, partial removal of calcium ions with citrate did not increase the plastic, elastic or viscoelastic components even when the residual calcium was reduced to 3.5% of the exchange capacity. The breaking stress of the fibres was rather more sensitive to calcium removal, being reduced by 50% at 7% calcium saturation. CDTA‐extracted fibres broke by cell separation at very low stress. These characteristics did not appear compatible with removal of calcium ions, or their displacement by protons, as a mechanism for auxin‐induced growth in this material: however such mechanisms are not excluded in other tissues or under other conditions. Strong chelating agents which remove enough calcium to weaken cell walls should be avoided in experiments on other mechanisms of auxin‐induced growth.