Abstract
A study involving 36 wether lamb carcasses was conducted in order to evaluate the effects of 0.3 M CaCl 2 injection on final tenderness in muscle Longissimus thoracis et lumborum. Injection of wether lamb carcasses with CaCl 2 accelerated the post mortem tenderization process. Both control and distilled water injected animals had similar Ca 2+-dependent proteases (μ-calpain and m-calpain) and their inhibitor (calpastatin) activities, whereas these were all significantly decreased in CaCl 2 injected animals. The effect of CaCl 2 injection on tenderness was recorded by measuring shear force values 2 and 6 days post mortem. Tenderness was significantly improved by CaCl 2 injection at both at 2 and 6 days post mortem. It was concluded that activation of μ-calpain and m-calpain was responsible for the observed post mortem proteolysis and tenderization.
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