Tenacibaculum lutimaris sp. nov., isolated from a tidal flat in the Yellow Sea, Korea

Abstract

Four Gram-negative, rod-shaped bacterial strains, TF-26(T), TF-28, TF-42 and TF-53, were isolated from a tidal flat in the Yellow Sea, Korea, and their taxonomic positions were determined by a polyphasic characterization. The strains grew optimally in the presence of 2-3 % (w/v) NaCl and at 30-37 degrees C. The predominant menaquinone detected in the four strains was MK-6. These strains contained large amounts of fatty acids C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH, iso-C(15 : 0), iso-C(16 : 0) 3-OH, C(15 : 0) and iso-C(17 : 0) 3-OH. The DNA G+C contents of the four strains were 32.3-32.8 mol%. Strains TF-26(T), TF-28, TF-42 and TF-53 showed 16S rRNA gene sequence similarity levels of 99.8-100 % and DNA-DNA relatedness levels of 82-87 %. The four strains exhibited 16S rRNA gene sequence similarity levels of 95.0-98.0 % to the type strains of the five current Tenacibaculum species, and DNA-DNA relatedness levels between the four strains and two phylogenetic relatives, Tenacibaculum mesophilum DSM 13764(T) and Tenacibaculum skagerrakense DSM 14836(T), were less than 21 %. On the basis of phenotypic, phylogenetic and genetic data, strains TF-26(T), TF-28, TF-42 and TF-53 were classified in the genus Tenacibaculum as members of a novel species, for which the name Tenacibaculum lutimaris sp. nov. (type strain, TF-26(T)=KCTC 12302(T)=DSM 16505(T)) is proposed.