Abstract

AbstractFreshwater pearl mussel, Hyriopsis cumingii, is the most economically important mussel species in China. Due to overexploitation and changes of water quality, H. cumingii is facing serious population decline and local extinction. We have isolated and characterized 10 polymorphic microsatellite loci from a genomic DNA library enriched for CA and AG repeats with the aim of developing a set of codominant DNA markers for analysing genetic diversity and population structure of this species. The average allele number of the 10 markers was 7.8 per locus, ranging from two to 16 in 24 unrelated individuals. Nine of 10 markers conformed to Hardy–Weinberg equilibrium and inherited independently, suggesting these microsatellites could be useful for studying population genetics, designing conservation strategies and developing breeding programs.

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