Abstract
Ten polymorphic microsatellite loci were isolated from the Atlantic Silverside (Menidia menidia) in order to test hypotheses regarding the role of adaptive phenotypic variation in structuring estuarine populations along coastal North America. Loci were amplified in three multiplex panels requiring a total of four individual PCRs. All loci were highly polymorphic in individuals screened from an estuary in Nova Scotia, and none exhibited significant departures from Hardy–Weinberg equilibrium. The results suggest that these loci will be sensitive to low levels of neutral divergence among populations across M. menidia populations.
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