Abstract
We isolated and characterized 10 microsatellite loci from the western mosquitofish, Gambusia affinis. Loci were screened in 30 individuals from a single location from their native range in coastal Texas. The number of alleles per locus ranged from 4 to 25, observed heterozygosity ranged from 0.40 to 0.93, and the probability of identity values from 6.0 × 10−3 to 2.7 × 10−1, with an overall PI for all loci of 1.3 × 10−13. These new loci provide tools for examining the genetic diversity, structure, and pedigree G. affinis populations.
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