Abstract

Ten novel microsatellite loci were isolated from an enriched genomic library from the striped skunk (Mephitis mephitis) to aid in landscape genetics studies and rabies virus control planning. Average observed and expected heterozygosities were 0.653 and 0.744, respectively. Mean number of alleles was 7.8 (ranging from 2 to 11). Two loci were not in Hardy–Weinberg equilibrium, likely due to the presence of null alleles. No evidence of linkage disequilibrium was found between the loci. Cross-species amplification tests indicated all markers successfully amplified and were polymorphic in the closely related hooded skunk (Mephitis macroura), while its applicability to other mephitids varied.

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