Temporally structured impulse activity in spontaneously discharging somatosensory cortical neurons in the awake cat:: recognition and quantitative description of four different patterns of bursts, post-recording GFAP immunohistology and computer reconstruction of the studied cortical surface

Abstract

We elaborated two methods used in two previous publications [J. Martinson, H.H. Webster, A.A. Myasnikov, R.W. Dykes, Recognition of temporally structured activity in spontaneously discharging neurons in the somatosensory cortex in waking cats, Brain Res. 750 (1997) 129–140 [16]; H.H. Webster, I. Salimi, A.A. Myasnikov, R.W. Dykes, The effects of peripheral deafferentation on spontaneously bursting neurons in the somatosensory cortex of waking cats, Brain Res. 750 (1997) 109–121 [21]]: (A) a procedure for detecting and classifying brief epochs of high-frequency extracellular impulse activity (bursts) recorded chronically in the somatosensory cortex of the awake cat, and (B) a modification of an immunohistochemical technique [L.A. Bevento, L.B. McCleary, An immunochemical method for marking microelectrode tracks following single-unit recordings in long surviving, awake monkeys, J. Neurosci. Meth. 41 (1992) 199–204 [5]] for visualization of electrode tracks and electrolytic lesions around the tip of tungsten-in-glass microelectrodes [D.M.D. Landis, The early reactions of non-neuronal cells to brain injury, Annu. Rev. Neurosci. 17 (1994) 133–151 [15]] weeks after lesions were made in cortex. The burst recognition and classification method uses an interval threshold to determine the beginning and end of one epoch [M. Armstrong-James, K. Fox, Effects of ionophoresed noradrenaline on spontaneous activity of neurons in rat primary somatosensory cortex, J. Physiol. (London), 335 (1983) 427–447 [3]] in the original sequence of interspike intervals (ISIs) to segregate and analyze separately a burst. The threshold is based on the duration of the shortest modal ISI found in the autocorrelogram [J. Martinson, H.H. Webster, A.A. Myasnikov, R.W. Dykes, Recognition of temporally structured activity in spontaneously discharging neurons in the somatosensory cortex in waking cats, Brain Res. 750 (1997) 129–140 [16]]. The technique allowed recognition of bursts with several distinctive patterns: (i) an initial, longer ISI followed by progressively shorter ones; (ii) an initially shorter ISI followed by progressively longer ones; (iii) patterns where the intermediate ISI could be either longer or shorter than surrounding ones; and (iv) consecutive ISIs of relatively equal duration. Among the cells discharging in bursts with equal ISIs, the technique allows recognition of cells generating only short (up to three to five intervals) bursts, and others generating mixtures of a short and long (up to six or more intervals) bursts. Finally, frequency distributions of the probability of encountering bursts having intervals of a stated length is described. The visualization of tracks from chronic recording experiments is important for relating neuronal function to a specific cytoarchitectural region and a specific cortical layer. Several modifications of the procedure of immunostaining for GFAP allows identification of recording sites in clearer relationship to the cytoarchitectonic details of cat somatosensory cortex. Themes: Sensory systems Topics: Somatosensory cortex and thalamocortical relationships