Abstract
The petrochemical industry has played a considerable role in generation and release of waste in the environment. Activated sludge and facultative lagoons are commonly used for domestic and industrial wastewater treatment due to their low-cost and minimal need for operational requirements. Microorganisms present in wastewater treatment plant (WWTP) are responsible for most nutrient removal. In this study, microbiological and physicochemical parameters were used to estimate changes in bacterial community in a petrochemical industrial WWTP. The activated sludge was the place with higher heterotrophic bacterial quantification. Denitrifying bacteria was reduced at least 5.3 times throughout all collections samples. We observe a decrease in the total Kjeldahl nitrogen, oxygen demand and phosphate throughout the WWTP. In this work, we also use Matrix-Assisted Laser Desorption Ionisation-Time-of-Flight Mass Spectrometry (MALDI-TOF MS) for bacteria isolates identification comparing with 16S rDNA sequencing. The MALDI-TOF MS allowed the identification of 93% of the isolates and only 5% show different results from 16S rDNA sequencing showing that the MALDI-TOF MS can be a tool for identifying environmental bacteria. The observation of microbial community dynamics in the WWTP is important in order to understand the functioning of the ecological structure formed in a specific environment.
Highlights
Petrochemical industry processing is responsible for the generation of large volumes of effluents characterized by contaminants such as iron, suspended solids, oils and fats, and hydrocarbons, among other inorganic ions (Wang et al 2014)
The biological process occurs with the coexistence of cyanobacteria and heterotrophic bacteria
With the exception of sulphate-reducing bacteria, activated sludge was the site with higher e20180394 14 | 18
Summary
Petrochemical industry processing is responsible for the generation of large volumes of effluents characterized by contaminants such as iron, suspended solids, oils and fats, and hydrocarbons, among other inorganic ions (Wang et al 2014). According to the National Water Agency from Brazil - ANA (Agencia Nacional das Águas 2013), Biological treatment processes in the form of wastewater treatment plants (WWTPs) are the most widely used approach for treating municipal and industrial wastewater due to the high efficiency for organic matter removal and the low operational cost (Ye and Zhang 2013). MALDI-TOF MS has gained popularity as a microbial biotyping tool due to its speed, lowcost, simplicity, and applicability for a wide range of microbes. It is becoming an increasingly essential technique for microbial characterization and identification in environmental microbiology and microbial diversity studies (Patil et al 2015, Kopcakova et al 2014, Busquets et al 2014). MALDITOF MS showed a high potential for the screening and discrimination of different environmental bacterial (Koubek et al 2012)
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