Temporal variation in the prevalence of the crayfish plague pathogen,Aphanomyces astaci, in three Czech spiny-cheek crayfish populations

Abstract

North American crayfish species are natural hosts of the crayfish plague pathogen Aphanomyces astaci . The spiny-cheek crayfish Orconectes limosus , widespread in Central Europe, is the main reservoir of A. astaci in Czech Republic. We tested if there are temporal changes in the prevalence of infected individuals (i.e. , the proportion of individuals in which the pathogen is detected) in spiny-cheek crayfish populations. Crayfish from three populations shown previously to be infected to different extents (high, intermediate and low), were repeatedly sampled in different years (2004–2010) and seasons. The presence of A. astaci in the soft abdominal crayfish cuticle was tested by specific amplification of the pathogen DNA. There was no substantial temporal variation in pathogen prevalence in the highly and very lowly infected populations. However, a significant long-term as well as seasonal decrease was found in the intermediately infected population. This decline could be related to a decrease in population density over the studied years, and to crayfish seasonal moulting, respectively. A reliable estimate of pathogen prevalence in American crayfish populations thus requires repeated monitoring over years, preferably during the same season before the main period of crayfish moulting.