Temporal variation in substratum-specific rates of N uptake and metabolism and their contribution at the stream-reach scale

Abstract

AbstractWhole-stream rates of nutrient uptake and metabolism vary with season in forested headwater streams, but seasonal variation in rates at the substratum scale, which collectively generate whole-stream patterns, have not previously been quantified. We measured gross primary production (GPP), community respiration (CR), and NO3− uptake rates (NO3− U) on 7 substrata using in situ microcosms during spring, summer, and autumn in 3 headwater streams in northern Michigan. We quantified the areal reach coverage of each substratum, scaled NO3− U and metabolism to the stream reach (100 m), and compared scaled NO3− U to whole-stream NO3− U (measured using short-term nutrient additions). Patterns of GPP, CR, and NO3− U were different among substratum types and seasons. Substratum-specific NO3− U was positively related to GPP on epilithic biofilms and to CR on epixylic biofilms, bryophytes, and coarse and fine benthic organic matter (FBOM). During summer, large wood and FBOM accounted for 86% of reach-scale NO3−...