Temporal response properties of lumbar-projecting vestibulospinal neurons to roll tilt in decerebrate cats

Abstract

In decerebrate cats, rotation about the longitudinal axis of the animal, leading to sinusoidal stimulation of labyrinth receptors, produces a tonic contraction of limb extensors during side-down tilt (alpha responses) and of dorsal neck extensors during side-up tilt (beta responses). These changes in posture are mediated, at least in part, by lateral vestibular nucleus (LVN) neurons, with response characteristics to stimulation of macular and/or canal receptors that have so far been evaluated at the level of either unidentified vestibulospinal (VS) neurons or vestibulo-collic neurons projecting to the upper cervical cord. In the present study we investigated the dynamics of the responses of VS neurons projecting to the lumbosacral segments of the spinal cord to increasing frequencies of tilt (from 0.026 to 0.32 Hz, +/- 10 degrees). All the recorded units showed an average phase lead with respect to position of +25.6 +/- 5.5 degrees (SE) at the tilt frequency of 0.026 Hz. Most of these neurons (n = 32) were particularly activated during side-down tilt (alpha responses) and showed either a stable phase or an increase in phase lead of the responses with increasing frequency of tilt. At the tilt frequency of 0.026 Hz, the smaller the phase lead of the responses, the larger was the response gain. Moreover, the smaller the phase lead of the responses at that frequency of tilt, the smaller the increase in gain but the larger was the increase in lead of the responses obtained by increasing the stimulation frequency up to 0.32 Hz. Through this set of finely organized changes in unit response characteristics, the overall output of this population of neurons increased, while the phase angle of the responses reached the mean value of +64.9 +/- 2.6 degrees (SE), thus becoming more related to the velocity than to the positional signal. The remaining units (n = 7), which were mainly activated during side-up tilt (beta responses), displayed an increase in phase lag of the responses to increasing frequency of stimulation, which reached the mean value of -118.9 +/- 14.5 degrees (SE) at 0.32 Hz. The differences in the dynamic properties of these VS neurons projecting to the lumbosacral cord, with respect to those of previously recorded populations of VS neurons, are discussed.