Temporal relationships between food acquisition and voluntary exercise in mice

Abstract

Patterns of operant food acquisition in a closed economy and bouts of either voluntary wheel running (WR) or spontaneous locomotor activity in a standard condition (SC) with no wheel were examined in young adult male and female C57BL/6 mice across a range of nose poke prices (FUP) per food pellet. Both sexes showed vigorous WR or locomotor activity. At each FUP, WR groups had higher food intake than SC groups. Despite substantially higher mean body weight of males compared with females, intakes and activity did not differ by sex in the SC groups and males lost weight more rapidly as FUP increased. In contrast, WR males ran ∼33% further per day than females, increased their food intake (above that of SC counterparts) more than females, and lost less body weight than SC males. By parsing the night in four 3h epochs it was found that food intake declined progressively through the night in both WR and SC mice and that the hyperphagia of WR relative to SC groups was most evident early in the night, coincident with highest activity. No large or systematic sex differences were revealed in these temporal analyses. Analysis of data at 60s resolution showed that pellet acquisition occurred in many small or short bouts, the timing of which was either intercalated or concurrent with either locomotor activity or WR. The results show that increased eating due to WR occurs concurrently with maximum running, and with no evidence of delayed compensation.